منابع مشابه
Visualization of acidic organelles in intact cells by electron microscopy.
We report the synthesis of a probe that permits the visualization by electron microscopy of acidic organelles in intact cells. This probe, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP), is a basic congener of dinitrophenol that readily diffuses into intact cells. Its primary and tertiary amino groups (apparent pKa, 10.6) allow it to be concentrated in acidic organelles and to be ...
متن کاملMembrane-coating granules are acidic organelles which possess proton pumps.
Lysosomes are by definition organelles that maintain an internal acidic pH and contain hydrolytic enzymes. Membrane-coating granules contain a battery of hydrolytic enzymes, in addition to their lamellar discs, and are therefore commonly assumed to be lamellate lysosomes. Although there are data confirming the existence of enzymes in membrane-coating granules, there is no direct evidence to sug...
متن کاملLegionella pneumophila Replication Vacuoles Mature into Acidic, Endocytic Organelles
After ingestion by macrophages, Legionella pneumophila inhibits acidification and maturation of its phagosome. After a 6-10-h lag period, the bacteria replicate for 10-14 h until macrophage lysis releases dozens of progeny. To examine whether the growth phase of intracellular L. pneumophila determines the fate of its phagosome, interactions between the endosomal network and pathogen vacuoles we...
متن کاملEndomembrane organelles with an acidic lumen Visualization of the acidic luminal pH of endomembrane organelles The acidic luminal pH of the endomembrane organelles can be visualized using pH-sensitive fluorescent dyes, such as Acridine Orange and fluorescein isothiocyanate (FITC)-
bounded) organelles are involved in the dynamic membrane trafficking processes essential for protein sorting and targeting, receptor-mediated endocytosis, neural or hormonal signal transduction and other processes (Mellman et al., 1986; Goldstein et al., 1985). These organelles include the Golgi apparatus, secretory vesicles, coated vesicles, endosomes, lysosomes and synaptic vesicles. Their lu...
متن کاملcAMP-dependent recruitment of acidic organelles for Ca2+ signaling in the salivary gland.
Autonomic neural activation of intracellular Ca2+ release in parotid acinar cells induces the secretion of the fluid and protein components of primary saliva critical for maintaining overall oral homeostasis. In the current study, we profiled the role of acidic organelles in shaping the Ca2+ signals of parotid acini using a variety of imaging and pharmacological approaches. Results demonstrate ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2020
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2020.183458